BIOFACT

PRODUCT

HiGene™ Genomic DNA Prep Kit [Column Type]

Isolation of Pure Genomic DNA from Various Samples

  • BIOFACT

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    Cat. No. Product Size
    GD141-050 HiGene™ Genomic DNA Prep Kit (For Whole Blood, Bacterium, Plant, Animal Tissue, Fungus) 50 prep
    GD141-100 HiGene™ Genomic DNA Prep Kit (For Whole Blood, Bacterium, Plant, Animal Tissue, Fungus) 100 prep
    GD183-550 HiGene™ Proteinase K, 20mg/ml - freezing dry 550 ㎕
    GD181-500 HiGene™ Lysozyme, 100 ㎎ / ㎖ - freezing dry 500 ㎕
    GD282-400 HiGene™ RNase A, 4 ㎎ / ㎖ - freezing dry 400 ㎕
    PE290-25h HiGene™ RNase A(100mg/ml) - solution type(냉동) 1.25 ㎖ X 2 ea
    GD142-050 HiGene™ Genomic DNA Prep Kit For Yeast 50 prep
    GD142-100 HiGene™ Genomic DNA Prep Kit For Yeast 100 prep
    GD286-400 HiGene™ Lyticase & Buffer, 2.5 unit/㎕ - freezing dry(enzyme) 400 ㎕

  • Description

    HiGene™ Genomic DNA Prep Kit [Column type] is designed to isolate the genomic DNA using glass microfiber membrane as a spin column type. The kit provides pure and high quality genomic DNA preparation system by removing PCR inhibition substances such as divalent cations and proteins. HiGene™ Genomic DNA Prep Kit [Column type] is using convenient method for the isolation of pure genomic DNA directly from various samples including blood, bacteria, plants and others.

    ■  Feature
    • Spin column type
    • Membrane : Glass microfiber
    • Column binding capacity : 20 ㎍ DNA / Column 

    ■  Application
    • PCR (Real-Time PCR, Multiplex PCR, Long PCR and etc.)
    • Southern blotting analysis
    • Clone library construction
    • Genotyping (SNP, SSR, AFLP, T-RFLP, etc.)

  • Workflow

     - HiGene™ Genomic DNA Prep Kit [GD141-100]

  • QC Data


     
    Know-how for High yield 
    1. Please use recommended volume / amount of each sample. In case of isolation from large amount of sample, adjust the input volume of each solution in proportion to the amount of sample.
    2. Prepare 80% Ethanol (not provided) in a WB Bottle. Make it fresh before use. 
    3. Please remove EtOH completely thorough column centrifugation before using DNA Hydration Solution.
    4. Add dried enzyme to D.W (Buffer) and mix well. Please store it at -20°C.
    5. Please use the product according to its expiration date.
    6. Please do not vortexing strongly to avoid DNA degradation during cell lysis step.
    7. Storage of GD1 at lower temperature may cause precipitation of salts due to SDS. In this case, please use it after thawing in the microwave/dry oven.
    8. Elution efficiency can be increased when using DNA Hydration Solution after pre-heating at 50 °C for 10 minutes. (Especially, in case of large DNA fragment)

  • Document

  • Etc.

    Reference
    1.  LI, Mei Jia, et al. Characterization and Pathogenicity of Alternaria vanuatuensis, a New Record from Allium Plants in Korea and China. Mycobiology, 2014, 42.4: 412-415.
    2.  BACK, Chang-Gi, et al. Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences. The plant pathology journal, 2015, 31.3: 212.
     
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