BIOFACT

PRODUCT

BioFACT™ T4 DNA Ligase

Suitable Enzyme for All Common DNA Ligations

  • BIOFACT

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    Cat. No. Product Size
    DL101-40k BioFACT™ T4 DNA Ligase 40,000 U
  • Description

    BioFACT™ T4 DNA ligase, widely used in both DNA repair and DNA replication, is isolated from a recombinant E.coli strain and catalyzes he formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. It can join blunt-end and cohesive-end termini (Including sticky, ends for TA Cloning) as well as repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.  

    Feature
    • Source : Bacteriophage T4

     Application
    • Joining of double-stranded DNA with cohesive or blunt end
    • Joining of linker or adaptors to blunt-ended DNA
    • Repairing nicks in duplex DNA, RNA or DNA-RNA hybrids
    • Inversed PCR product
    • Sub-cloning
    • Cloning of restricted DNA fragments into a plasmid