BIOFACT

PRODUCT

BioFACT™ Gel & PCR Purification System

Gel Extraction and PCR Purification in one Kit

  • BIOFACT

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    Cat. No. Product Size
    GP104-100 HiGene™ Gel & PCR Purification System 100 prep
    GP104-200 HiGene™ Gel & PCR Purification System 200 prep

  • Description

    BioFACT™ Gel & PCR Purification System is a spin column typed kit which has dual functions of gel extraction and PCR Purification. It provides the high purification system of high yield DNA from 100 bp to 14 kb, and it has an advantage of selective purification system of the two conditions (high yield and primer-dimer removal) according to the application needs. BioFACT™ Gel & PCR Purification System is suitable to obtain high quality DNA fragments through high salt buffer and glass microfiber membrane. Furthermore, it is available to verify whether Help B has been properly used in process of PCR purification with naked eye as pH Indicator in UB buffer turns purple by use of Help B.



    ■  Feature
    • Spin column type
    • Membrane : Glass fiber
    • Column binding capacity : 20 ㎍ DNA / column
    • Purification size : 100 bp ~ 14 kb
    • Recovery yield : above 80% (70 bp~5 kb)
    • Sequencing grade
    • pH Indicator included
    • Visual check to help B treatment

    ■  Application
    • General sequencing             
    • Restriction enzyme digestion             
    • Cloning

  • Workflow

    - BioFACT™ Gel & PCR Purification System [GP104-100]

     
    ※ UB buffer contains pH Indicator which turns purple when the pH changes due to issues with expiration date or storage condition.
       Make sure to check the color before use as UB buffer with purple colored may decrease yield. 

    ※ In case UB mixture (PCR product + UB) is added to spin column treated with Help B buffer, the mixture that passed through spin column turns purple.
       Users can easily check with the naked eye whether spin column has been treated with Help B buffer.
     

  • QC Data

  • Etc.

    Reference
    1.  WIN, Theint Theint, et al. Monitoring the microbial community shift throughout the shock changes of hydraulic retention time in an anaerobic moving bed membrane bioreactor. Bioresource technology, 2016, 202: 125-132
    2.  LEE, Seung-Yeol, et al. Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR. The plant pathology journal, 2014, 30.4: 445.
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