BIOFACT

PRODUCT

BioFACT™ Bst DNA Polymerase

DNA Polymerase for Isothermal Amplification

  • Standard

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    Cat. No. Product Size
    BP101-16h BioFACT™ Bst DNA Polymerase, Large Fragment 1,600 U (8 U/㎕)
    BP101-80h BioFACT™ Bst DNA Polymerase, Large Fragment 8,000 U (1,600 U x 5 ea)

  • Description

    BioFACT™ Bst DNA Polymerase is a recombinant protein lacking the 5'→3' exo-nuclease activity of Bacillus stearothermophilus DNA Polymerase. The optimum conditions for the product are 65℃, 30 to 60 minutes, and it can be applied to Loop-Mediated isothermal amplification (LAMP) PCR with isothermal amplification technology.

    ■  Feature
    • Synthesis primer : gene specific primer
    • Suitable for applications requiring thermophilic strand displacement
    • optimization activity at 65℃
    • Relative activity 80~90% at 60℃, 65~75% at 55℃, 15~20% at 50℃
    • lacking 5´→3´ exonuclease activity
    • 5' →  3' exonuclease activity : No
    • 3' →  5' exonuclease activity (fidelity) : No

    ■  Application
    • Loop-Mediated isothermal Amplification(LAMP)
    • Isothermal Amplification
    • Whole genome amplification
    • RT-LAMP

  • QC Data

    -  Comparison of LAMP PCR activity by concentration of enzyme between BioFACT™ Bst DNA Polymerase and other company’s polymerase 
     

     
    -  Comparison of sensitivity according to various template concentrations between BioFACT™ Bst DNA Polymerase and other companies’ polymerases 
     
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