BIOFACT

PRODUCT

BioFACT™ Uracil DNA Glycosylase For PCR Diagnosis

Elimination of Carry-Over Contamination

  • BIOFACT

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    Cat. No. Product Size
    UD121-10h BioFACT™ Uracil-DNA Glycosylase 1,000 U
  • Description

    BioFACT™ Uracil-DNA Glycosylase is useful to reduce the carry-over contamination in PCR based diagnostics. In UDG system, PCR is performed using dUTP through substitution of dTTP and post-PCR is performed after UDG treatment. If there is contamination of PCR product in the previous step, by the UDG system, contaminated DNA is eliminated through hydrolysis of N-glycosylic bond between Uracil and template in PCR product. It can’t be used for PCR enzymes which have proofreading activity such as BioFACT™ Pfu DNA Polymerase, BioFACT™ Lamp Taq DNA Polymerase and BioFACT™ Lamp Pfu DNA Polymerse. 

      Feature
    • Source : E.coli (K-12 strain)
    • Removing uracil-containing DNA
    • Elimination of carry-over contamination

      Application
    • Diagnosis system using PCR