BIOFACT

PRODUCT

BioFACT™ RNase Inhibitor

Efficient Protection of RNA from Ribonuclease

  • BIOFACT

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    Cat. No. Product Size
    RI152-20h BioFACT™ RNase Inhibitor 2,000 U (40 U/㎕)

  • Description

    BioFACT™ RNase Inhibitor is a 91 KDa recombinant protein derived from Rattus norvegicus. It inhibits RNase activity through a 1:1 non-covalent binding with RNase A, B and C, and does not affect the activity of RNase H, T1, S1 nuclease, SP6 RNA polymerase, T7 RNA polymerase, T3 RNA polymerase, AMV reverse transcriptase, M-MLV Reverse transcriptase, and Taq DNA polymerase. 
    BioFACT™ RNase Inhibitor is useful for protecting RNA from ribonuclease during cDNA synthesis, one-step RT-PCR, RNA synthesis, and RNA preparation experiments. 

    ■  Feature
    • 1 Unit : Activity that inhibits 50% of the activity of 5 ng of RNase A
    • Source : An E.coli strain with a recombinant RNase Inhibitor gene from Rattus
    • Molecular weight : 91 kDa Protein 
    • Heat inactivation : 70 ℃ for 10 min 
    • Optimal pH range : pH 5.5 ~ 9.0  

    ■  Application
    • Isolation and purification of RNA 
    • RT-PCR reactions 
    • cDNA synthesis reactions.
    • In vitro transcription/translation reactions 
    • RNase protection assays 
    • RNA-dependent in vitro functional assays

  • QC Data

    -  Comparison of LOD between BioFACT™ RNase Inhibitor and other company’s RNase Inhibitor (Concentration of RNase A is fixed to 1.6 ng)  
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