BIOFACT

PRODUCT

5X Band Helper™

PCR Additives for Difficult PCR

  • BIOFACT

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    Cat. No. Product Size
    BB741-10k 5X Band Helper™ 1 ㎖ × 10 ea
  • Description

    5X Band Helper™ is an innovative PCR additive which is highly suitable for use in all PCR enzymes of BIOFACT. It enables efficient amplification and improved results can be obtained by adjusting of 5X Band Helper™ in the following cases. 

    * Difficult templates which are GC-rich or have a high degree of secondary structure
    * Presence of non-specific bands or products
    * Please refer to the protocol of PCR Enzymes to check the recommended concentrations of 5X Band Helper™.
      It is recommended to use a minimum amount of 5X Band Helper™ in case of PCR enzymes that have fidelity features.

     Application
    • PCR additive for optimal PCR reaction set up
    • Increase of amplification efficiency using BioFACT™ PCR enzymes

    Set-up of 5X Band Helper™ 
    Please perform PCR amplification by adjusting the final concentration of 5X Band Helper™ from 0.5X to 2X and choose the optimal concentration of 5X Band Helper™. 

    How to use Band Helper™
  • QC Data

    -  Comparison of PCR amplification efficiency on various concentrations of 5X Band Helper™




    -  Comparison of Gradient PCR amplification efficiency on various concentrations of 5X Band Helper™