BIOFACT

PRODUCT

BioFACT™ OneStep RT-PCR Series ⓗ

Fast and convenient OneStep RT-PCR reaction in a single tube

  • Standard

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    Cat. No. Product Size
    BR861-050 BioFACT™ One Step RT-PCR Kit ⓗ, Enzyme mixture, 5X Reaction Buffer (Including 10mM dNTP), 5X Band Helper™ 50 reaction
    BR861-100 BioFACT™ One Step RT-PCR Kit ⓗ, Enzyme mixture, 5X Reaction Buffer (Including 10mM dNTP), 5X Band Helper 100 reaction

  • Master Mix

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    Cat. No. Product Size
    BR331-10h BioFACT™ 2X OneStep RT-PCR Master Mix ⓗ, with dye 1 ㎖ x 1 ea
    BR331-50h BioFACT™ 2X OneStep RT-PCR Master Mix ⓗ, with dye 1 ㎖ x 5 ea

  • Pre-Mix

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    Cat. No. Product Size
    BR431-096 BioFACT™ 2X OneStep RT-PCR Pre-Mix ⓗ, with dye, total 30㎕ reactions 96 Tubes (8 strip x 12 ea)

  • Description

    BioFACT™ OneStep RT-PCR Series is designed for convenience and high efficiency in carrying out RT-PCR in a single tube at one step. Included BioFACT™ RTase and BioFACT™ H-Star Taq DNA Polymerase is blended in one mixture with best optimized rate to simplify reaction step and reducing the possibility of contamination. 
    BioFACT™ OneStep RT-PCR Series has great first-strand cDNA yields by its thermostable characteristic at 50°C for activation of BioFACT™ RTase and it allows robust, high specific amplification by BioFACT™ H-Star Taq DNA Polymerase included in the mixture. It is optimized to amplify less than 1 kb size target.

    ■  Feature
    • Thermostable and RNase H variant of M-MLV RTase (RNase H-)
    • cDNA synthesize at 42℃–50℃ (highest activity at 50℃)
    • Synthesis of long length cDNA (＜14 kb)
    • Synthesis gene size : < 1 kb PCR
    • Synthesis primer : gene specific primer
    • Chemical mediated Hot start enzyme 
    • Great efficiency & high specificity
    • Easy sample optimization
    • Low-copy transcripts amplification

    ■  Application
    • RT-PCR

  • QC Data

    -  Comparison of amplification efficiency on various RNA concentrations between BioFACT™ OneStep RT-PCR Kit and other companies’ RT-PCR Kits

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