BIOFACT

PRODUCT

BioFACT™ Pfu DNA Polymerase

High Fidelity / Amplification of Blunt-End Fragment

  • Standard

    * 좌우로 스크롤하시면 확인가능합니다. * Scroll left & right
    Cat. No. Product Size
    PD116-250 BioFACT™ Pfu DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.3 ㎖) 250 U
    PD116-500 BioFACT™ Pfu DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.6 ㎖) 500 U
    PD116-25h BioFACT™ Pfu DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 3 ㎖) 2,500 U (500 U × 5 ea)
    PD116-50h BioFACT™ Pfu DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 6 ㎖) 5,000 U (500 U × 10 ea)

  • Master Mix

    * 좌우로 스크롤하시면 확인가능합니다. * Scroll left & right
    Cat. No. Product Size
    PD301-50h BioFACT™ 2X Pfu PCR Master Mix 1, w/o Band Helper™, with dye 1 ㎖ × 5 ea
    PD302-50h BioFACT™ 2X Pfu PCR Master Mix 2, with 0.5X Band Helper™, with dye 1 ㎖ × 5 ea

  • Pre-Mix

    * 좌우로 스크롤하시면 확인가능합니다. * Scroll left & right
    Cat. No. Product Size
    PD401-096 BioFACT™2X Pfu PCR Pre-Mix, w/o Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
    PD401-480 BioFACT™2X Pfu PCR Pre-Mix, w/o Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)

  • Description

    BioFACT™ Pfu DNA Polymerase is recombinant enzyme derived from Pyrococcus furiosus and expressed in E. coli. It has superior thermostability and possesses 3' to 5' exonuclease proofreading activity which results in PCR fragments with fewer errors. PCR fragments generated by BioFACT™ Pfu DNA Polymerase are blunt-ended and it can be available for blunt end cloning using All in One™ PCR Cloning Kit without additional A-tailing step. 

    ■  Feature
    • Source : Pyrococcus furiosus 
    • 5' →  3' exonuclease activity : No
    • 3' →  5' exonuclease activity (fidelity) : Yes
    • Amplification size : < 3 kb PCR 
    • A-tailing : No
    • Error rate : 1-2 bp error / 106 bp
    • 12-fold higher PCR fidelity over Taq DNA Polymerases

    ■  Application
    • High fidelity PCR          • Site-directed mutagenesis          • Blunt end form cloning

  • QC Data

    -  Comparison of amplification efficiency according to various sizes of template between BioFACT™ A-Star LampTaq DNA Polymerase and other companys’ Pfu DNA Polymerases
     

     

    -  Comparison of fidelity for various PCR enzymes
     
LIST