BIOFACT

PRODUCT

BioFACT™ Lamp Taq DNA Polymerase

Accurate Long range PCR with Low Error Rate

  • Standard

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    Cat. No. Product Size
    LT116-250 BioFACT™ Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.3 ㎖) 250 U
    LT116-500 BioFACT™ Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.6 ㎖) 500 U
    LT116-25h BioFACT™ Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 3 ㎖) 2,500 U (500 U × 5 ea)
    LT116-50h BioFACT™ Lamp Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 6 ㎖) 5,000 U (500 U × 10 ea)

  • Master Mix

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    Cat. No. Product Size
    LT301-50h BioFACT™ 2X Lamp Taq PCR Master Mix 1, w/o Band Helper™, with dye 1 ㎖ × 5 ea
    LT302-50h BioFACT™ 2X Lamp Taq PCR Master Mix 2, with 0.5X Band Helper™, with dye 1 ㎖ × 5 ea

  • Pre-Mix

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    Cat. No. Product Size
    LT402-096 BioFACT™ 2X Lamp Taq PCR Pre-Mix, with 0.5X Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
    LT402-480 BioFACT™ 2X Lamp Taq PCR Pre-Mix, with 0.5X Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)

  • Description

    BioFACT™ Lamp Taq DNA Polymerase contains a blend of Taq DNA Polymerase and Pfu DNA polymerase which exhibits a 3´-exonuclease activity (proofreading). It is highly suitable for PCR amplification of long DNA fragments from 5 to 20 kb and it exhibits low error rate due to its proofreading activity (week). BioFACT™ Lamp Taq DNA Polymerase provides increased and higher yields than that which can be achieved with Taq DNA polymerase through its superior amplification efficiency and it generates PCR products with A-tailing, used for TA cloning.

    ■  Feature
    • Source : Pyrococcus furiosus & Thermus aquaticus 
    • 5' →  3' exonuclease activity : Yes
    • 3' →  5' exonuclease activity (fidelity) : Yes (weak)
    • Amplification size : <40 kb, based on λDNA
    • Hot start activity: No 
    • A-tailing : Yes
    • Error rate : 5 - 6 bp error/ 106 bp

    ■  Application
    • Long range PCR (<40 kb, based on λDNA)  
    • High productivity PCR
    • Inverse PCR 
    • TA cloning
    • Colony PCR

  • QC Data

    -  PCR amplification data for various gene sizes between BioFACT™ Lamp Taq DNA Polymerase and other companies’ PCR enzymes
     
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