BIOFACT

PRODUCT

BioFACT™ H-Star Taq DNA Polymerase

Highly Specific Amplification of Less Than 1 kb Size Target DNA

  • Standard

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    Cat. No. Product Size
    HS106-250 BioFACT™ H-Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.2 ㎖) 250 U
    HS106-10h BioFACT™ H-Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.8 ㎖) 1,000 U (250 U x 4 ea)

  • Master Mix

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    Cat. No. Product Size
    HS301-40h BioFACT™ 2X H-Star Taq PCR Master Mix 1, w/o Band Helper™ , with dye 1 ㎖ × 4 ea
    HS303-40h BioFACT™ 2X H-Star Taq PCR Master Mix 2, with 1X Band Helper™ , with dye 1 ㎖ × 4 ea
    HS341-40h h BioFACT™ 2X H-Star Taq PCR Master Mix 1, w/o Band Helper™, with dye (UDG System) 1 ㎖ × 4 ea

  • Pre-Mix

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    Cat. No. Product Size
    HS401-096 BioFACT™ 2X H-Star Taq PCR Pre-Mix, w/o Band Helper™,with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
    HS401-480 BioFACT™ 2X H-Star Taq PCR Pre-Mix, w/o Band Helper™,with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)

  • Description

    BioFACT™ H-Star Taq DNA Polymerase is a chemical-mediated Hot Start Taq DNA polymerase which leads to complete inactivation of the polymerase until the initial heat activation step. Through this feature, it provides high PCR specificity, avoiding extension of non specific annealed primers or primer-dimers and it is highly suitable for efficient amplification of difficult templates caused by high GC content or complex structure. BioFACT™ H-Star Taq DNA Polymerase is optimized to amplify less than 1 kb size target DNA and it can be supplemented by increasing the cycle as amplification efficiency and productivity of chemical-mediated Hot Start PCR enzymes may be lower than general Taq DNA Polymerase.

    ■  Feature
    • Source : Thermus aquaticus 
    • 5' →  3' exonuclease activity : Yes
    • 3' →  5' exonuclease activity (fidelity) : No
    • Amplification size : < 1 kb PCR
    • Hot start activity (Chemical-mediated hot start) 
    • A-tailing : Yes
    • Error rate : 12 - 13 bp error / 106 bp

    ■  Application
    • Hot start PCR                • TA cloning         
    • High specificity PCR    • Real-Time PCR         
    • Multiplex PCR               • Allele specific PCR
    • High G+C rich / Repeat region PCR          
    • Genotyping                 • Suitable for UDG System

  • QC Data

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