BIOFACT

PRODUCT

BioFACT™ Thumb Taq DNA Polymerase

Taq DNA polymerase with Increased PCR efficiency

  • Standard

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    Cat. No. Product Size
    TT116-500 BioFACT™ Thumb Taq DNA polymerase with 10 mM dNTP Mix (each 10 mM, 0.4 ㎖) 500 U

  • Master Mix

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    Cat. No. Product Size
    TT301-50h BioFACT™ Thumb Taq PCR Master Mix 1, w/o Band Helper™, with dye 1 ㎖ × 5 ea
    TT302-50h BioFACT™ Thumb Taq PCR Master Mix 2 with 0.5X Band Helper™, with dye 1 ㎖ x 5 ea

  • Pre-Mix

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    Cat. No. Product Size
    TT402-096 BioFACT™ Thumb Taq PCR Pre-Mix, with 0.5X Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
    TT402-480 BioFACT™ Thumb Taq PCR Pre-Mix, with 0.5X Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)

  • Description

    BioFACT™ Thumb Taq DNA Polymerase is a widely used polymerase product most commonly used in the traditional PCR with applications in general PCR such as colony PCR as well as other various types of PCR methods for diagnosis and testing. Due to high amplification efficiency compared to Taq DNA polymerase, a large amount of PCR products can be obtained. 

    ■  Feature
    • Source : Thermus aquaticus 
    • 5' →  3' exonuclease activity : Yes
    • 3' →  5' exonuclease activity (fidelity) : No
    • Amplification size : < 5 kb PCR 
    • A-tailing : Yes
    • Hot start activity : No 
    • Error rate : 12 - 13bp error / 106 bp

    ■  Application
    • Routine PCR
    • SNP / Genotyping
    • TA Cloning

  • QC Data

    - Comparison of enzyme activity by various enzyme units between BioFACT™ Taq and BioFACT™ Thumb Taq 



    - Comparison of amplification efficiency by various template concentrations between BioFACT™ Taq and BioFACT™ Thumb Taq

  • Etc.

    Reference
    1. Bioresour Technol. 2015 Dec 5;202:125-132. doi: 10.1016/j.biortech.2015.11.085. [Epub ahead of print] Monitoring the microbial community shift throughout the shock changes of hydraulic retention time in an anaerobic moving bed membrane bioreactor. Win TT1, Kim H2, Cho K2, Song KG3, Park J4
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