BIOFACT

PRODUCT

BioFACT™ OneStep qRT-PCR Master Mix (For Intercalating Dye)

One-step RT-qPCR using RNA as a Template

  • Master Mix

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    Cat. No. Product Size
    RQ351-10h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SYBR® Green I in mixture, w/o Rox reference dye 1 ㎖ x 1 ea
    RQ351-50h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SYBR® Green I in mixture, w/o Rox reference dye 1 ㎖ x 5 ea
    RQ352-10h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SYBR® Green I in mixture, Low Rox 1 ㎖ x 1 ea
    RQ352-50h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SYBR® Green I in mixture, Low Rox 1 ㎖ x 5 ea
    RQ353-10h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓐ including SYBR® Green I in mixture, with high Rox reference dye 1 ㎖ x 1 ea
    RQ353-50h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓐ including SYBR® Green I in mixture, with high Rox reference dye 1 ㎖ x 5 ea
    RQ361-10h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SFCgreen® I in mixture, w/o Rox reference dye 1 ㎖ x 1 ea
    RQ361-50h BioFACT ™ 2X OneStep qRT-PCR Master Mix ⓗ including SFCgreen® I in mixture, w/o Rox reference dye 1 ㎖ x 5 ea

  • Description

    BioFACT™ 2X Multi-Star OneStep qRT-PCR Master Mix (For Intercalating dye) is an optimized product which allows the qRT-PCR to be done in one PCR tube by creating an enzyme mixture with Thermostable BioFACT™ RTase and H-Star(A-Star) Taq DNA polymerase, which has Hot Start PCR features. Intercalating dye can be selected from either SFCgreen® I or SYBR® Green I, and it can be conveniently used simply by adding the primer and the template in order to perform PCR. This product can be used with all types of Real-Time PCR instruments. 

    ■  Feature
    • High sensitivity using BioFACT™ H-Star(A-Star) Taq DNA polymerase
    • Pre-denaturation time : 15 mins. 
    • Enzyme mixture of BioFACT™ RTase and H-Star(A-Star) Taq DNA Polymerase)
    • Intercalating dye : SYBR® Green I & SFCgreen® I

    ■  Application
    • Quantification of target RNA using RT-PCR
    • Various applications using qPCR  

  • QC Data

    -  Comparison of sensitivity by product type (Limit of Detection)
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