BIOFACT

PRODUCT

20X EvaGreen™

Intercalating Dye with High Sensitivity and Stability

  • BIOFACT

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    Cat. No. Product Size
    31000-B500 20X EvaGreen™ 500 ㎕

  • Description

    20X EvaGreen™ is the next generating DNA binding dye that can be used in Real-Time PCR. EvaGreen™ is compatible with HRM analysis as it has less PCR interference, such as slowing down the primer dimer formation and extension reaction, as well as high sensitivity to double-stranded DNA, and can produce more accurate results. It has little PCR inhibition even at high concentrations and is compatible with most qPCR instruments in a wavelength similar to SYBR® Green I. It is non-mutagenic, noncytotoxic, and very stable under various PCR conditions.

    ■  Application
    • λabs/λem = 500/530 nm (DNA bound)
    • λabs = 471 nm (w/o DNA)
    • Similar wavelength with that for SYBR®green I or FAM 
    • Non-mutagenic, Non-cytotoxic
    • Less PCR inhibition by dye concentration 
    • Superior bright fluorescence & Long half-life
    • HRM / Melting curve analysis available  

    ■  Comparison test between EvaGreen™ and SYBR® Green I

  • Etc.

    Reference
    1. Plant Physiol Biochem. 2015 Sep;94:19-27. doi: 10.1016/j.plaphy.2015.05.003. Epub 2015 May 9. Transgenic poplar expressing Arabidopsis YUCCA6 exhibits auxin-overproduction phenotypes and increased tolerance to abiotic stress. Ke Q1, Wang Z2, Ji CY1, Jeong JC1, Lee HS1, Li H2, Xu B2, Deng X2, Kwak SS3.
    2. Ke, Qingbo, et al. "Transgenic poplar expressing codA exhibits enhanced growth and abiotic stress tolerance." Plant Physiology and Biochemistry (2016)
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