BIOFACT

PRODUCT

LyoFACT™ OneStep RT-PCR Pre-Mix ⓗ

High sensitive & High specific RT-PCRs convenient in single tubes

  • Pre-Mix

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    Cat. No. Product Size
    BR531-096 LyoFACT™ OneStep RT-PCR Pre-Mix ⓗ Lyophilisates, with dye 96 Tubes (8 strip × 12 ea)
  • Description

    LyoFACT™ OneStep RT-PCR Pre-Mix is a lyophilized type designed for convenience and high efficiency in carrying out RT-PCR in a single tube at one step. Included BioFACT™ RTase and BioFACT™ H-Star Taq DNA Polymerase is blended in one mixture with best optimized rate to simplify reaction step and reducing the possibility of contamination. LyoFACT™ OneStep RT-PCR Pre-Mix has great first-strand cDNA yields by its thermostable characteristic at 50℃ for activation of BioFACT™ RTase and it allows robust, high specific amplification by BioFACT™ H-Star Taq DNA Polymerase included in the mixture. LyoFACT™ OneStep RT-PCR Pre-Mix offers higher sensitivity as it can increase the amount of template than solution-typed PCR Pre-Mix.

    ■  Feature
    • Thermostable and RNase H variant of M-MLV RTase (RNase H-
    • Synthesize cDNA at 42℃ – 50℃ 
    • Synthesis gene size : < 1 kb PCR
    • cDNA synthesis & PCR in one sing tube
    • Great first-strand cDNA yields & Hot-Start activity 
    • Especially the highest activity at 50℃ 
    • Synthesis primer : gene specific primer 
    • High Specificity & Easy sample optimization 
    • Low-copy transcripts amplification

    ■  Application
    • RT-PCR
  • QC Data