BIOFACT
PRODUCTLyoFACT™ OneStep RT-PCR Pre-Mix ⓗ
High sensitive & High specific RT-PCRs convenient in single tubes
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Pre-Mix
* 좌우로 스크롤하시면 확인가능합니다. * Scroll left & rightCat. No. Product Size BR531-096 LyoFACT™ OneStep RT-PCR Pre-Mix ⓗ with dye 96 Tubes (8 strip × 12 ea) -
Description
LyoFACT™ OneStep RT-PCR Pre-Mix is a lyophilized type designed for convenience and high efficiency in carrying out RT-PCR in a single tube at one step. Included BioFACT™ RTase and BioFACT™ H-Star Taq DNA Polymerase is blended in one mixture with best optimized rate to simplify reaction step and reducing the possibility of contamination. LyoFACT™ OneStep RT-PCR Pre-Mix has great first-strand cDNA yields by its thermostable characteristic at 50℃ for activation of BioFACT™ RTase and it allows robust, high specific amplification by BioFACT™ H-Star Taq DNA Polymerase included in the mixture. LyoFACT™ OneStep RT-PCR Pre-Mix offers higher sensitivity as it can increase the amount of template than solution-typed PCR Pre-Mix.
■ Feature
• Thermostable and RNase H variant of M-MLV RTase (RNase H-)
• Synthesize cDNA at 42℃ – 50℃
• Synthesis gene size : < 1 kb PCR
• cDNA synthesis & PCR in one sing tube
• Great first-strand cDNA yields & Hot-Start activity
• Especially the highest activity at 50℃
• Synthesis primer : gene specific primer
• High Specificity & Easy sample optimization
• Low-copy transcripts amplification
■ Application
• RT-PCR -
QC Data