BIOFACT

PRODUCT

LyoFACT™ RT Pre-Mix

Lyophilized typed RT Pre-Mix including primers

  • Pre-Mix

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    Cat. No. Product Size
    BR546-096 LyoFACT™ RT Pre-Mix (dT18 Plus) Lyophilisates 96 Tubes (8 strip × 12 ea)
    BR547-096 LyoFACT™ RT Pre-Mix (dN9 Plus) Lyophilisates 96 Tubes (8 strip × 12 ea)
    BR548-096 LyoFACT™ RT Pre-Mix (dT18/dN9 Plus) Lyophilisates 96 Tubes (8 strip × 12 ea)

  • Description

    LyoFACT™ RT Pre-Mix is based on recombinant type of M-MLV reverse transcriptase(RNase H-) expressed in E. coli which has greater first-strand cDNA yields than existing wild type of reverse transcriptase due to inhibition of RNA's secondary structure by its thermostable characteristic at 50℃ for activation. LyoFACT™ RT Pre-Mix is a lyophilized type designed to increase storage stability and it is supplied as a ready-to-use, 2X concentrated Pre-Mix which includes an optimized mixture of all the reagents such as RTase, RNase inhibitor, DTT and dNTPs in a 0.2 ㎖ 8-strip tube. In addition, Oligo dT (dT18) or Random Oligo (dN9) are included on the kit and it can be used by adding template RNA & RNase free water only. It offers higher sensitivity as it can increase the amount of template than solution-typed PCR Pre-Mix. 

    ■  Feature
    • Thermostable and RNase H variant of M-MLV RTase (RNase H-)   
    • Synthesize cDNA at 42℃ – 50℃   
    • Especially the highest activity at 50℃ 
    • Synthesis of long length cDNA (＜14 kb) 
    • Synthesis primer : random primer, oligo-d(T), gene specific primer 
    • Inhibition of RNA's secondary structure 
    • Oligo(dT)18 and(or) random hexamer primers provided

    ■  Application
    • First-strand cDNA synthesis      • RT-PCR      • Real-Time PCR      • Primer extension analysis
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