BIOFACT

PRODUCT

LyoFACT™ PCR Pre-Mix

Lyophilized typed PCR Pre-Mix

  • Pre-Mix

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    Cat. No. Product Size
    ST501-096 LyoFACT™ Taq PCR Pre-Mix Lyophilisates, with dye 96 Tubes (8 strip × 12 ea)
    MS501-096 LyoFACT™ Multi-Star PCR Pre-Mix Lyophilisates, with dye 96 Tubes (8 strip × 12 ea)

  • Description

    LyoFACT™ PCR Pre-Mix is designed to increase storage stability by freeze-drying solution-typed PCR Pre-Mix. Its ready to use format can be stored at room temperature for long-term storage while remaining structurally and chemically stable as the moisture of it is decreased to less than 1%, unlike the wet dry formulation. In addition, it is fully reconstituted after addition of water (rehydration).

    ■  Feature
    • Source : Thermus aquaticus             
    • 5' → 3' exonuclease activity : Yes       
    • 3' → 5' exonuclease activity (Fidelity) : No
    • Amplification size : Taq < 5kb, Multi-Star <1kb           
    • Hot start activity : Taq  (No), Multi-Star (Yes(chemical-mediated)) 
    • 12-13 bp error / 106 bp         
    • A-tailing : Yes           
    • Amplification of more than one target in a single reaction (Multi-Star)
     
    ■  Application
    <Taq>
    • Routine PCR    • PCR screening   • TA cloning   • Colony PCR
    <Multi-Star>
    • Multiplex PCR ( ~ 13 bands in a parallel)
    • Allele specific PCR ( ~ 21 bands in a parallel)
    • Methylation specific PCR     • SNP detection
    • General sequencing            • TA cloning
    • SSR / MS         • UDG system 

  • QC Data

    -  Stability test on various periods (Changes in cake shape)
              

     
      - Comparison of activity test according to various periods between LyoFACT™ PCR Pre-Mix and other lyophilized typed PCR Mix 
              
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