BIOFACT

PRODUCT

BioFACT™ 2X Multi-Star PCR Series

Optimal PCR Kit for Multi Band Amplification

  • Master Mix

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    Cat. No. Product Size
    MS301-25h BioFACT™ 2X Multi-Star PCR Master Mix, with dye 0.5 ㎖ × 5 ea
    MS302-25h BioFACT™ 2X Multi-Star PCR Master Mix, w/o dye 0.5 ㎖ × 5 ea

  • Pre-Mix

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    Cat. No. Product Size
    MS401-096 BioFACT™ 2X Multi-Star PCR Pre-Mix, with dye, total 30 ㎕ reactions 96 Tubes (8 strip × 12 ea)
    MS402-096 BioFACT™ 2X Multi-Star PCR Pre-Mix, w/o dye, total 30 ㎕ reactions 96 Tubes (8 strip × 12 ea)

  • Description

    Multiplex PCR is a simple, efficient and powerful PCR technique that allows simultaneous amplification of two or more target genes in parallel in a single tube. BioFACT™ 2X Multi-Star PCR Series is available in a convenient ready-to-use Master Mix / Pre-Mix format which contains Hot Start PCR enzyme and optimized buffer which minimize inhibition of different primer sets in same tube during reaction. Included BioFACT™ H-Star Taq DNA Polymerase which is chemical-mediated Hot Start DNA polymerase enables high specific and accurate detection by inhibition of non specific amplification and primer-dimer due to its automatic heat-activation characteristics. BioFACT™ 2X Multi-Star PCR Series is highly suitable for research laboratories, forensic, diagnostic genotyping applications where simultaneous analysis of multiple markers is required. 

    ■  Feature
    • Source : Thermus aquaticus 
    • 5' →  3' exonuclease activity : Yes
    • 3' →  5' exonuclease activity (fidelity) : No
    • Amplification size : < 1 kb PCR 
    • Hot Start activity (Chemical-mediated Hot Start) : Yes
    • A-tailing : Yes
    • Pre-denaturation time: 15 mins. 

    ■  Application
    • Multiplex PCR ( ~ 13 bands in a parallel) 
    • Allele specific PCR ( ~ 21 bands in a parallel) 
    • Methylation specific PCR
    • SNP detection
    • General sequencing
    • TA cloning
    • SSR / MS
    • Suitable for UDG system

  • QC Data

    -  Comparison of amplification efficiency on various primer sets between BioFACT™ 2X Multi-Star PCR Series and other Company’s multiplex PCR enzyme
     


    - Single / multiplex PCR
     
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