PRODUCT
제품소개BioFACT™ Bst DNA Polymerase
Isothermal Amplification을 위한 DNA Polymerase
옵션
- Standard
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Description
BioFACT™ Bst DNA Polymerase는 Bacillus stearothermophilus DNA Polymerase의 5'→3' exo-nuclease activity가 결여된 재조합 단백질입니다. 제품의 최적 조건은 65℃, 30~60 분이며, 등온증폭(Isothermal Amplification) 기술이 적용된 Loop-Mediated isothermal Amplification(LAMP) PCR에 적용 가능한 제품 입니다.
■ Feature
• Synthesis primer : gene specific primer
• Suitable for applications requiring thermophilic strand displacement
• optimization activity at 65℃
• Relative activity 80~90% at 60℃, 65~75% at 55℃, 15~20% at 50℃
• lacking 5´→3´ exonuclease activity
• 5' → 3' exonuclease activity : No
• 3' → 5' exonuclease activity (fidelity) : No
• 다양한 진단 실험에 활용
■ Application
• Loop-Mediated isothermal Amplification(LAMP)
• Isothermal Amplification
• Whole genome amplification
• RT-LAMP
• Various diagnosis applications
• Assay development for pathogen detection -
QC Data
- 제조사별 Enzyme 농도별 LAMP PCR 활성 비교
- Template 농도에 따른 제조사별 민감도 비교
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Document
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PROTOCOL (MANUAL GUIDE)
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MSDS (MATERIAL SAFETY DATA SHEET)
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